Correlation of retinal
Ultra-High Optical Coherence Tomography Imaging
with Histo- and Zytomorphology
Ultrahigh resolution optical coherence tomography allows to
directly visualize the microarchitecture of living retinas in situ.
(A) pancorrected UHR OCT of distal retina
(B) high resolution OCT
(C) immunolabeled (anti-S-opsin) human cone photoreceptor from cryosection
CB: cone cell body; LM: external limiting membrane; MC: miicrovillous müller cell tips at ELM; IS: inner segment; ELL: ellipsoid =mitochondrial region of IS; MY: myoid= cytoplasmatic (+Golgi app etc) region if IS; OS: outer segment, cmv: calycal microvilli at IS-OS transition; PE: pigment epithelium;
FERNANDEZ, E. J., et al. Ultrahigh resolution optical coherence
tomography and pancorrection for cellular imaging
of the living human retina.
Opt Express, 16, 11083-94 (2008).
We participated in as series of comparative studies
learning to identify the retinal sublayers in OCTs of model species
by correlating them to the corresponding elements in histological preparations.