Limitations of the simple RC-Filter/Op-amp Circuit
Although this simple circuit worked (in most cases!), I think you'll agree
that the quality of the ECG was pretty poor. Hopefully, you noticed the
following problems:
-
There was still too much noise from AC hum
-
Although the QRS complex could just be seen, the P and T wave were swamped
by noise
-
Heavier smoothing (by reducing the filter cutoff) reduced the noise, but
also reduced the ECG signal
-
There was also a problem of drift
-
There was often a DC offset on the signal due to static charge on the subject
-
There was a low frequency drift ("baseline wander") whenever the subject
or electrodes moved (motion artefact)
-
It made it difficult to keep the signal on the oscilloscope screen
-
It could even saturate the amplifier by making the output rise or fall
to the supply rail voltage
The Differential Amplifier
In order to improve the signal further, we need a new design for our amplifier:

This new design is called a differential amplifier, because
it amplifies the difference between the two input voltages.
The gain is given by
Gain = R2.(V2-V1)
R1
Common mode rejection
Since the output is proportional to the difference between the two voltages,
anything (e.g. noise) which is present on both inputs will be cancelled
out. However, a signal (e.g. the EKG) which is different on the two inputs
will be amplified, which of course is exactly what we want. The ratio of
the gain of the difference gain to the common gain (usually expressed in
dB) is called the Common Mode Rejection Ratio (CMRR).
CMRR = Differential Gain
Common Gain
An typical differential amplifie has a CMRR of about 30,000. So, supposing
we build a circuit with a differential gain of 1,000, this means that the
common gain (acting on the noise) will be:
Differential Gain / Common Gain= 30,000
So,
Common Gain = Differential Gain / CMRR
= 1,000/30,000 = 1/30 or 0.03
In other words, instead of getting amplified, the noise will actually
be attentuated 30-fold.
CMRR in dB
Just to be awkward, gains and CMRR are usually quoted in dB, so for voltage
gains, the equation becomes:
CMRR (dB) = 20 log (Differential Voltage Gain / Common Voltage Gain)
Thus, a typical differential amp will have a CMRR of 20 log 30,000 =
90 dB
How about going the other way? Well, the inverse of a log to base 10
is 10 raised to its power, so:
Voltage Gain ratio = 10CMRR/20
e.g. 90 dB = 1090/20 , i.e. a voltage gain ratio of 104.5
or 30,000
The Instrumentation Amplifier
Unfortunately, the differential amplifier turns out to be rather limited
in its performance because of the low input impedance of (R2
+ R1). To improve this, two bootstrapped buffer amplifiers
(which are just op-amps with unity gain) are commonly added, which results
in the simple instrumentation amplifier:

In practice, it is difficult to precisely match resistors that are discrete
components. To overcome this problem the entire circuit is put on
a single integrated circuit, since IC manufacturing technology enables
precise resistor ratios to be obtained. Such chips as Analog Devices AD620
find widespread use in working with low-level signals with large common-mode
components in noisy environments - just the sort of situation we find in
biomedical engineering.
AC coupling
The other problems we had were with DC offset, drift and motion artefact.
All of these problems are caused by very low-frequencies (DC is zero frequency),
so we need to use a high-pass filter with a very low cutoff frequency.
We need to keep the cutoff frequency very low to avoid degrading the ECG
signal, so the capacitor needs to be large (0.47 or 1 uF) and the resistor
similarly large (e.g. 1 MW).
So, our final circuit is:
The AD620 Instrumentation amp
Luckily, we don't have to build all of this because commercial instrumentation
amplifiers are available. The AD620 has a CMRR of 100 dB with a differential
gain that is adjustable up to 1,000. This is done by changing the value
of a resistor, RG on the input of the chip. Here's the datasheet.
By buying a commercial amplifier we also don't need to worry about the
offset null because the internbal circuits is perfectly balanced in the
factory.

The
REF pin is yet another name for ground.