Handbook of Culture Media for Food and Water Microbiology

Handbook of Culture Media for Food and Water Microbiology 3rd Edition:

Janet E L Corry (Editor), Gordon D W Curtis (Editor), R M Baird (Editor)
ISBN: 978-1-84755-916-6 , Extent: 636, Price:  £149.95

 This is the highly anticipated third edition of a book written by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene. It is a handy reference for microbiologists wanting to know which media to use for the detection of various groups of microbes in foods or water, and how to check the performance of the media. The information and methods described in the first two editions of this book have been widely adopted by practical microbiologists as well as many microbiological media manufacturers. The latest edition concentrates on media for water as well as food microbes - selecting those which have been evaluated and shown to function optimally, and contains a series of chapters on media for different groups of microbes, written by experts from all over the world. This book will prove to be an indispensible resource for academics, postgraduate students and all those working in industry related areas.

M. Manafi: Chapter 12: Culture Media for Detection and Enumeration of “Total” Enterobacteriaceae, Coliforms and Escherichia coli from Foods, Handbook of Culture Media for Food and Water Microbiology (3), 2011, 233-260

IN VITRO (Issue 2/2008),

Manafi M. 2015 (Book chapter) Detection of Specific Taxa Using Chromogenic and Fluorogenic Media, p 2.1.1-1-2.1.1-9. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, 4th Edition. ASM Press, Washington, DC.

 image of Manual of Environmental Microbiology, 4th Edition

PUBLICATION (2013):  Schwameis R, Erdogan-Yildirim Z, Manafi M, Zeitlinger MA, Strommer S, Sauermann R.: Effect of pulmonary surfactant on antimicrobial activity in vitro. Antimicrob  Agents Chemother. 2013,  57 :5151-4.

Manafi M, Waldherr K, Kundi M., Evaluation of CP Chromo Select Agar for the enumeration of Clostridium perfringens from water. Int J Food Microbiol. 2013, 167: 92-95 


Schwameis R, Fille M, Manafi M, Zeitlinger M, Sauermann R.. Enhanced activity of linezolid against Staphylococcus aureus in cerebrospinal fluid. Res Microbiol. 2012 163:157-60.

PUBLICATION (2011):  Burian, A., C. Wagner, J. Stanek, M. Manafi, M. Bohmdorfer, W. Jager and M. Zeitlinger. Plasma protein binding may reduce antimicrobial activity by preventing intra-bacterial uptake of antibiotics, for example clindamycin. J Antimicrob Chemother. 66: 134-137 (2011)

Erdogan-Yildirim, Z., A. Burian, M. Manafi and M. Zeitlinger. Impact of pH on bacterial growth and activity of recent fluoroquinolones in pooled urine. Res Microbiol. 162: 249-252 (2011)

Jantschitsch, C. T. K., M. Manafi, M. Safer, and A. Tanew. Scombroid Fish Poisoning - An Underrecognized Dermatologic Emergency. J.of Am. Acad. of Dermatology. 65:246-247(2011).

PUBLICATION (2010):  Goshu, G., D. Byamukama, M. Manafi, A. K. T. Kirschner and A. H. Farnleitner. A pilot study on anthropogenic faucal pollution impact in Bahir Dar Gulf of Lake Tana, northern Ethiopia. Ecohydrology and Hydrobiology. 10: 271-280 (2010)

PUBLICATION (2009):  Orenga, S., A. L. James, M. Manafi, J. D. Perry and D. H. Pincus. Enzymatic substrates in microbiology. J Microbiol Methods. 79: 139-155 (2009).

González González, M. I. Torres Rojas, T. S. Chiroles Rubalcaba and M. Manafi. Medios de cultivo fluorogénicos y cromogénicos y su evaluación en aguas de consumo y costeras. Hig. Sanid. Ambient 9: 422-430 (2009). (PDF)


international Workshop

TEHRAN 2006: HACCP IN FOOD PRODUCTION+Rapid Testing Methods for Food Pathogens;  


JAPAN 2005: Innovative microbiology testing with chromogenic  and fluorogenic substrates and media (

Evaluation and comparison of LB-BGLB method and 4 commercially media for the detection of total coliforms and E. coli in water after chlorination



  • Fluorogenic and Chromogenic Substrates Used in Bacterial Diagnostic.  Microbiol. Rev. 55:335-348, (1991)

  • Fluorogenic and chromogenic substrates in culture media and identification tests. Int. J. Food Microbiol.31:45-58, (1996)  

  • Use Of Enzyme Tests In Characterization and Identification Of Aerobic and Facultative Anaerobic Gram Positive Cocci J- Clinical Microbiol. Rev.11:318-340 (1998)

  • Culture media containing fluorogenic and chromogenic substrates. De Ware (n)-Chemicus 28:12-17 (1998)

  • Evaluation of CHROMagar Candida for rapid  screening of clinical specimens for Candida species  Mycoses, 42: 61-65 (1999).

  • Quantitative determination of total coliforms and Escherichia coli in marine waters with chromogenic and fluorogenic media. J. Appl. Bacteriol. 88, 280-285 (2000).

  • New developments in chromogenic and fluorogenic culture media. Int J Food Microbiol 2000  60:205-218 (2000)

Double left-click on the video for Fullscreen: Readycult Coliforms P/A test for Detection and Identification of Coliform bacteria and E.coli in Finished waters (EPA-approved)