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KRAGLER LAB


Friedrich Kragler
group leader

tel. 43-1-4277 52815
 
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Members

Rainer Leitner
Shoudong Zhang

 

Regulation of Cell-To-Cell Transport of RNA and Proteins via Plasmodesmata

In plants homeotic proteins involved in meristem initiation and/or maintenance such as KNOTTED1 (KN1) or which confer and maintain floral meristem identity such as LEAFY are thought to move like viral movement proteins to adjacent cells via intercellular pores formed by plasmodesmata. A number of studies suggest that specific and regulated cell-to-cell transport of proteins and RNA molecules depends on cellular plasmodesmal pathway receptors. Homeotic transcription factors function within the nucleus, thus, a decision between non-cell-autonomy versus nuclear import has to be made within the cytoplasm. Next, actively transported transcription factors have to interact with specific receptors regulating access to the intercellular transport machinery established by plasmodesmata, which in turn transfers the non-cell-autonomous proteins to neighboring cells via the plasmodesmatal pores. However, limited knowledge is available regarding interaction partners of non-cell-autonomous transcription factors such as KN1-binding proteins and their functional role(s) in cellular distribution.

Recent results indicate that entry into the plasmodesmal transport pathway can be negatively regulated by a novel microtubules-associated protein named MPB2C. Structurally and functionally distinct proteins such as the tobacco mosaic virus movement protein (TMV-MP), KNOTTED1 (KN1) as well as the A. thaliana KN1 homologue SHOOT MERISTEMLESS (STM) interact with MPB2C. Ample presence of the MPB2C prevents cell-to-cell movement of the homeobox transcription factor KN1 and TMV-MP. In addition, we isolated a novel KN1/STM binding protein, KNB36, which is also transported from cell to cell and binds to MPB2C. To exclude that KN1 cell-to-cell transport is simply impaired due to the overexpression of an interacting protein we tested the effect of KNB36 on KN1 transport. In microinjection experiments KNB36 has no effect KN1 cell-to-cell transport. These observations suggest that MPB2C binds a range of structurally distinct proteins and specifically decides/regulates their intracellular distribution and, consequently, also access to the non-cell-autonomous transport pathway established by plasmodesmata.




Selected Publications

 

Yoo* B.-C., Kragler* F., Varkonyi-Gasic E., Haywood V., Archer-Evans S., Lee Y.-M., Lough, T.J., Lucas W.J. Systemic Small RNA Signaling System in Plants. Plant Cell, 2004, 1979-2000. (* equal contributions)

 

Kragler F., Trutnyeva K., Curin M., Gansch A., Waigmann E.

MPB2C, a Microtubule-Associated Plant Protein Binds to and Interferes with Cell-to-Cell Transport of Tobacco Mosaic Virus Movement Protein. Plant Physiology, 2003. 132: 1870-1883.

 

Aoki* K., Kragler* F., Xoconostle-C·zares B., Lucas W.J.

A Subclass of Plant Hsc70 Chaperones Carry a Motif that Mediates Trafficking through Plasmodesmata.

Proc. Nat. Acad. Sci. USA, 2002. 99:16342-16347. (* equal contributions)

Kunze M., Kragler F., Binder M., Hartig A., Gurvitz A.

Targeting of Malate Synthase 1 to the Peroxisomes of Saccharomyces cerevisiae Cells Depends on Growth on Oleic Acid Medium. European Journal of Biochemistry 2002. 269: 915-922.

 

Kragler F., Monzer J., Xoconostle-Cazares B., Lucas W.J.

Isolation and Characterization of Short Peptides that Function as Antagonists of Ribonucleoprotein Transport through Plasmodesmata. EMBO Journal, 2000. 19: 2856-2868.

 

Kragler* F., Christmann* J., Lametschwandtner* G., Hartig A., Harada J.J.

Identification and Analysis of the Plant Peroxisomal Targeting Signal 1 Receptor NtPEX5 NtPex5p from Nicotiana tabacum. Proc. Nat. Acad. Sci. USA, 1998. 95: 13336-13341. (* equal contributions)

 

Kragler F., Monzer J., Shash K., Xoconostle-Cazares B., Lucas W.J.

Cell-to-cell Transport of Proteins: Requirement for Unfolding and Characterization of Binding to a Plasmodesmal Receptor. The Plant Journal, 1998. 15: 367-381.

Brocard C., Kragler F., Simon M. M., Binder M., Hartig A.

The Tetratricopeptide Repeat-Domain of the PAS10 Protein of Saccharomyces cerevisiae is Essential for Binding the Peroxisomal Targeting Signal. Biochemical and Biophysical Research Communications, 1994. 204: 1016-1022.

 

Kragler F., Langeder A., Raupachova J., Binder M., Hartig A.

Two Independent Peroxisomal Targeting Signals in Catalase A of Saccharomyces cerevisiae. The Journal of Cell Biology, 1993. 120: 665-673.

Reviews:

Kragler F. Plasmodesmata - Protein Transport Signals and Receptors

Book Title: PLASMODESMATA, Chapter 3, Ed.: Karl Oparka (2005, in press).

 

Ruiz-Medrano R, Xoconostle-Cazares B, Kragler F.

The Plasmodesmatal Transport Pathway for Homeotic Proteins, Silencing Signals and Viruses.

Curr. Opin. Plant Biol., 2004, 7: 641-650.

 

Haywood, V., Kragler, F., Lucas W. J.

Plasmodesmata: Pathways for Macromolecular Signaling. Plant Cell, 2002, 14: S 303-325.

 

Lucas W.J., Yoo B.J., Kragler F.

RNA as a Long-Distance Information Macromolecule in Plants. Nature Reviews Molecular Cell Biology, 2001. 2: 849-857.

 

Kragler F., Lucas W.J., Monzer J.

Plasmodesmata: Dynamics, Domains and Patterning. Annals of Botany, 1998. 81: 1-10.

 

Other Publications:

Weisz H. and Kragler F. An Attempt at Sign-mediated Communication Theory. Journal of Global Ecology and Biogeography, 2002, 11:262-263. (Book Review)

Kragler F. and Mezitt L. Cosmic signals? Trends in Plant Science, 1997. 2: 246-247. (Meeting Review)




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