GERHARD WICHE, Ph.D.
Professor of Biochemistry and Molecular Cell Biology
phone +43-1-4277 52852
Fax +43-1-4277 52854
E-mail gerhard.wiche@univie.ac.at
Role of cytolinker proteins in morphogenesis, signalling and disease
Publications Gerhard Wiche
139. Winter L, Abrahamsberg C, Wiche G. (2008). Plectin isoform 1b-mediated mitochondrion-IF network linkage controls organelle shape. J Cell Biol e-pub.
138. Konieczny P, Fuchs P, Reipert S, Kunz WS, Zeöld A, Fischer I, Paulin D, Schröder R, Wiche G. (2008). Myofiber integrity depends on desmin network targeting to Z-disks and costameres via plectin isoforms. J Cell Biol 181, 667-681.
137. Reipert S, Kotisch H, Wysoudil B, Wiche G. (2008). Rapid Microwave Fixation of Cell Monolayers Preserves Microtubule-associated Cell Structures. J Histochem Cytochem e-pub.
136. Spazierer S, Raberger J, Gross K, Fuchs P, Wiche G. (2008). Stress-induced recruitment of epiplakin to keratin networks increases their resistance to hyperphosphorylation-induced disruption. J Cell Sci e-pub Feb 19.
135. Wally V, Klausegger A, Koller U, Lochmüller H, Krause S, Wiche G, Mitchell LG, Hintner H, Bauer JW. (2008). 5’ Trans-splicing repair of the PLEC1 gene. J Invest Dermatol 128, 568-574. E-pub 8 Nov 2007.
134. Konieczny P, Wiche G. (2007). Muscular integrity – a matter of interlinking distinct structures via plectin. In: Laing NG. (Ed.). “The Sarcomere and Skeletal Muscle Disease”. Landes Bioscience - Springer. (http://eurekah.com/chapter/3648). E-pub Oct. 2007.
133. Ackerl R, Walko G, Fuchs P, Fischer I, Schmuth M, Wiche G. (2007). Conditional targeting of plectin in prenatal and adult mouse stratified epithelia causes keratinocyte fragility and lesional epidermal barrier defects. J Cell Sci 120, 2435-2443.
132. Infante AA, Infante D, Chan MC, How PC, Kutschera W, Linhartová I, Müllner EW, Wiche G, Propst F. (2007). Ferritin associates with marginal band microtubules. Exp Cell Res 313, 1602-1614.
131. Rezniczek GA, Konieczny P, Nikolic B, Reipert S, Schneller D, Abrahamsberg C, Winder SJ, Wiche G. (2007). Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with β-dystroglycan. J Cell Biol 176, 965-977.
130. Spurny R, Abdoulrahman K, Janda L, Rünzler D, Köhler G, Castañón MJ, Wiche G. (2007). Oxidation and nitrosylation of cysteines proximal to the IF-binding site of plectin: effects of structure, vimentin-binding, and involvement in IF collapse. J Biol Chem 282, 8175-8187.
129. McMillan JR, Akiyama M, Rouan F, Mellerio JE, Lane EB, Leigh IM, Owaribe K, Wiche G, Fujii N, Uitto J, Eady RA, Shimizu H. (2006 ). Plectin defects in epidermolysis bullosa simplex with muscular dystrophy. Muscle Nerve 35, 24-35.
128. Osmanagic-Myers S, Gregor M, Walko G, Burgstaller G, Reipert S, Wiche G. (2006). Plectin-controlled keratin cytoarchitecture affects MAP kinases involved in cellular stress response and migration. J Cell Biol 174, 557-568.
127. Gregor M, Zeöld A, Oehler S, Andrä Marobela K, Fuchs P, Weigel G, Hardie G, Wiche G. (2006). Plectin scaffolds recruit energy-controlling AMP-activated protein kinase (AMPK) in differentiated myofibers. J Cell Sci 119, 1864-1875.
126. Tian R, Gregor M, Wiche G, Goldman JE (2006). Plectin regulates the organization of GFAP in Alexander Disease. Am J Pathol 168, 888-897.
125. Spazierer D, Fuchs P, Reipert S, Fischer I, Schmuth M, Lassmann H, Wiche G. (2006). Epiplakin is dispensable for skin barrier function and for integrity of keratin network cytoarchitecture in simple and stratified epithelia. Mol Cell Biol 26, 559-568.
124. Fuchs P, Spazierer D, Wiche G. (2005). Plectin rodless isoform expression and its detection in mouse brain. Cell Mol Neurobiol 25, 1141-1150.
123. Abrahamsberg C, Fuchs P, Osmanagic-Myers S, Fischer I, Propst F, Elbe-Bürger A, Wiche G. (2005). Targeted ablation of plectin isoform 1 uncovers role of cytolinker proteins in leukocyte recruitment. Proc Natl Acad Sci USA 102, 18449-18454.
122. Lesniewicz K, Lüscher-Firzlaff J, Poreba E, Fuchs P, Walsemann G, Wiche G, Lüscher B. (2005). Overlap of the gene encoding the novel poly(ADP-ribose) polymerase Parp10 with the plectin 1 gene and common use of exon sequences. Genomics 86, 38-46.
121. Rezniczek GA, Janda L, Wiche G. (2004). Plectin. Methods Cell Biol. 78, 721-755.
120. Fuchs P, Wiche G. (2004). Intermediate filament linker proteins: plectin and BPAG1. In: Encyclopedia of Biological Chemistry 2, 452-457. Academic Press. ISBN 0124437109.
119. Reipert S, Fischer I, Wiche G. (2004). High-pressure cryoimmobilization of murine skin reveals novel structural features and prevents extraction artifacts. Exp Dermatol 13, 419-425.
118. Koss-Harnes D, Høyheim B, Jonkman MF, de Groot WG, de Weerdt CJ, Nikolic B, Wiche G, Gedde-Dahl Jr. T (2004). Lifelong course and molecular characterization of the original Dutch family with Epidermolysis bullosa simplex with muscular dystrophy (EBS-MD). Homozygous novel plectin point mutation (E1914X). Acta Dermato-Venereol 84,124-131.
117. Ševčík J, Urbániková L, Koštan J, Janda L, Wiche G. (2004). Plectin actin-binding domain: crystal structure and binding to vimentin. Eur J Biochem 271, 1873-1884.
116. Osmanagic-Myers S, Wiche G. (2004). Plectin-RACK1 (receptor for activated C kinase 1) scaffolding: A novel mechanism to regulate PKC activity. J Biol Chem 279, 18701-18710.
115. Schara U, Tücke J, Mortier W, Nüsslein T, Rouan F, Pfendner E, Zillikens D, Bruckner-Tuderman L, Uitto J, Wiche G, Schröder R. (2004). Severe mucous membrane involvement in epidermolysis bullosa simplex with muscular dystrophy due to a novel plectin gene mutation. Eur J Pediatr 163, 218-222.
114. Reipert S, Fischer I, Wiche G. (2004). High-pressure freezing of epithelial cells on sapphire coverslips. J Microsc 213, 81-85.
113. Rezniczek GA, Abrahamsberg C, Fuchs P, Spazierer D, Wiche G. (2003). Plectin 5’-transcript diversity: short alternative sequences determine stability of gene products, initiation of translation, and subcellular localization of isoforms. Hum Mol Genet 12, 3181-3194.
112. Spazierer D, Fuchs P, Pröll V, Janda L, Oehler S, Fischer I, Hauptmann R, Wiche G. (2003). Epiplakin gene analysis in mouse reveals a single exon encoding a 725 kDa protein with expression restricted to epithelial tissues. J Biol Chem 278, 31657-31666.
111. House CM, Frew IJ, Huang HL, Wiche G, Traficante N, Nice E, Catimel B, Bowtell DD. (2003). A binding motif for Siah ubiquitin ligase. Proc Natl Acad Sci USA 100, 3101-3106.
110. Reipert S., Fischer I, Wiche G. (2003). Cryofixation of epithelial cells grown on sapphire coverslips by impact freezing. J Microsc 209, 76-80.
109. Andrä K, Kornacker I, Jörgl A, Zörer M, Spazierer D, Fuchs P, Fischer I, Wiche G. (2003). Plectin isoform-specific rescue of hemidesmosomal defects in plectin (-/-) keratinocytes. J Invest Dermatol 120, 189-197.
108. Hendrychová J, Vítová M, Bisová K, Wich G, Zachleder V. (2002). Plectin-like proteins are present in cells of Chlamydomonas eugametos (Volvocales). Folia Microbiol 47, 535-539.
107. Schröder R, Pacholsky D, Reimann J, Matten J, Wiche G, Fürst DO, van der Ven PF. (2002). Primary longitudinal adhesion structures: plectin-containing precursors of costameres in differentiating human skeletal muscle cells. Histochem Cell Biol 118, 301-310.
106. Lunter P, Wiche G. (2002). Direct binding of plectin to Fer kinase and negative regulation of its catalytic activity. Biochem Biophys Res Commun 296, 904-910.
105. Stroissnigg H, Repitz M, Miloloza A, Linhartova I, Beug H, Wiche G, Propst F. (2002). FIP-2, an IkappaB-kinase-gamma-related protein, is associated with the Golgi apparatus and translocates to the marginal band during chicken erythroblast differentiation. Exp Cell Res 278, 133-45.
104. Urbaniková L, Janda L, Popov A, Wiche G, Ševčík J. (2002). Purification, crystallization and preliminary X-ray analysis of the plectin actin-binding domain. Acta Crystallogr D Biol Crystallogr 58, 1368-70.
103. Schröder R, Kunz WS, Rouan F, Pfendner E, Tolksdorf K, Kappes-Horn K, Altenschmidt-Mehring M, Knoblich R, van der Ven PF, Reimann J, Fürst DO, Blumcke I, Vielhaber S, Zillikens D, Eming S, Klockgether T, Uitto J, Wiche G, Rolfs A. (2002). Disorganization of the desmin cytoskeleton and mitochondrial dysfunction in plectin-related epidermolysis bullosa simplex with muscle dystrophy. J Neuropathol Exp Neurol 61, 520-30.
102. Noiges R, Eichinger R, Kutschera W, Fischer I, Nemeth Z, Wiche G, Propst F. (2002). Microtubule-associated protein 1A (MAP1A) and MAP1B: light chains determine distinct functional properties. J Neurosci 22, 2106-2114
101. Koss-Harnes D, Høyheim B, Anton-Lamprecht I, Gjesti A, Jørgensen RS, Jahnsen FL, Olaisen B, Wiche G, Gedde-Dahl T. (2002). A site-specific plectin mutation causes dominant Epidermolysis bullosa simplex Ogna. Two identical de novo mutations. J Invest Dermatol 118, 87-93.
100. Janda L, Damborský J, Rezniczek GA, Wiche G. (2001). Plectin repeats and modules: strategic cysteines and their presumed impact on cytolinker functions. BioEssays 23, 1064-1069.
99. Bauer JW, Rouan F, Kofler B, Rezniczek GA, Kornacker I, Muss W, Hametner R, Klausegger A, Huber A, Pohla-Gubo G, Wiche G, Uitto J, Hintner H. (2001). A compound heterozygous one amino-acid insertion/nonsense mutation in the plectin gene causes Epidermolysis bullosa simplex with plectin deficiency. Am J Pathol 158, 617-625.
98. Meixner A, Haverkamp S, Wässle H, Führer S, Thalhammer J, Soares S, Nothias F, Kropf N, Bittner RE, Lassmann H, Wiche G, Propst F. (2000). MAP1B is required for axon guidance and is involved in the development of the central and peripherous nervous system. J Cell Biol 151, 1169-1178.
97. Carlsson L, Li Z, Paulin D, Price M, Breckler J, Robson R, Wiche G, Thornell L-E. (2000). Differences in the distribution of synemin, paranemin, and plectin in skeletal muscles of wild-type and desmin knock-out mice. Histochem. Cell Biol 114, 39-47.
96. Stegh A, Herrmann H, Lampel S, Weisenberger D, Andrä K, Seper M, Wiche G, Krammer PH, Peter ME. (2000). Identification of the cytolinker plectin as a major early in vivo substrate for caspase-8 during CD95- and tumor necrosis factor-receptor mediated apoptosis. Mol Cell Biol 20, 5665-5679.
95. Steinböck F, Nikolic B, Coulombe PA, Fuchs E, Traub P, Wiche G. (2000). Dose-dependent linkage, assembly inhibition, and disassembly of vimentin and cytokeratin 5/14 filaments through plectin's intermediate filament-binding domain. J Cell Sci 113, 483-491.
94. Kunz M, Rouan F, Pulkkinen L, Hamm H, Leschke R, Bruckner-Tuderman L, Bröcker E-B, Wiche G, Uitto J, Zillikens D. (2000). Epidermolysis bullosa simplex associated with severe mucous membrane involvement and novel mutations in the plectin gene. J Invest Dermatol 114, 376-380.
93. Fuchs P, Zörer M, Rezniczek GA, Spazierer D, Oehler S, Castañón MJ, Hauptmann R, Wiche G. (1999). Unusual 5' transcript complexity of plectin isoforms: novel tissue-specific exons modulate actin binding activity. Hum Mol Genet 8, 2461-2472.
92. Shimizu H, Masunaga T, Kurihara Y, Owaribe K, Wiche G, Pulkkinen L, Uitto J, Nishikawa T. (1999). Expression of plectin and HD1 epitopes in patients with epidermolysis bullosa simplex associated with muscular dystrophy. Arch Dermatol Res 291, 531-537.
91. Reipert S, Steinböck F, Fischer I, Bittner RE, Zeöld A, Wiche G. (1999). Association of mitochondria with plectin and desmin intermediate filaments in striated muscle. Exp Cell Res 252, 479-491.
90. De Pereda JM, Wiche G, Liddington RC.(1999). Crystal structure of the first pair of fibronectin type III modules from the cytoplasmic tail of integrin a6b4. EMBO J 18, 4087-4095.
89. Propst, F., and Wiche, G. (1999). Syncolin. In: Guidebook to the Cytoskeletal and Motor Proteins, eds. Kreis TE & Vale RD. Oxford University Press/Sambrook and Tooze Publishers, Oxford.
88. Wiche G. (1999). Plectin. In: Guidebook to the Cytoskeletal and Motor Proteins, eds. Kreis TE & Vale RD. Oxford University Press/Sambrook and Tooze Publishers, Oxford. p. 341-344.
87. Meixner A, Wiche G, Propst F. (1999). Analysis of the mouse MAP1B gene identifies a highly conserved 4.3 kb 3' untranslated region and provides evidence against the proposed structure of DBI-1 cDNA. Biochim Biophys Acta 1445, 345-350.
86. Tögel M, Eichinger R, Wiche G, Propst F. (1999). A 45 amino acid residue domain necessary and sufficient for proteolytic cleavage of the MAP1B polyprotein precursor. FEBS Lett 451, 15-18.
85. Steinböck FA, Wiche G. (1999). Plectin: a cytolinker by design. Biol Chem 380, 151-158.
84. Linhartová I, Repitz M, Dráber P, Nemec M, Wiche G, Propst F. (1999). Conserved domains and lack of evidence for polyglutmaine length polymorphism in the chicken homolog of the Machado-Joseph disease gene product ataxin-3. Biochim Biophys Acta 1444, 299-305.
83. Tögel M, Wiche G, Propst F. (1998). Novel features of the light chain of microtubule-associated protein MAP1B: microtubule stabilization, self interaction, actin filament binding, and regulation by the heavy chain. J Cell Biol 143, 695-707.
82. Andrä K, Stöcher M, Nikolic B, Drenckhahn D, Wiche G. (1998). Not just scaffolding: plectin regulates actin dynamics in cultured cells. Genes Dev 12, 3442-3451
81. Wiche G. (1998). Role of plectin in cytoskeleton organization and dynamics. J Cell Sci 111, 2477-2486.
80. Wiche G. (1998). Domain structure and transcript diversity of plectin. Biol Bull 194, 381-383.
79. Kutschera W, Zauner W, Wiche G, Propst F. (1998). The mouse and rat MAP1B genes: genomic organization and alternative transcription. Genomics 49, 430-436.
78. Rezniczek GA, de Pereda JM, Reipert S, Wiche G. (1998). Linking integrin a6b4-based cell adhesion to the intermediate filament cytoskeleton: direct interaction between the b4 subunit and plectin at multiple molecular sites. J Cell Biol 141, 209-225.
77. Tögel M, Wiche G, Propst F. (1998). Evidence against structural and functional identity of microtubule-associated protein MAP1B and proteoglycan claustrin. FEBS Lett 423, 254-258.
76. Mellerio JE, Smith FJD, McMillan JR, McLean EHI, McGrath JA, Morrison GAJ, Tierney P, Albert DM, Wiche G, Leigh IM, Geddes JF, Lane EB, Uitto J, Eady RAJ. (1997). Recessive Epidermolysis Bullosa Simplex associated with plectin mutations: infantile respiratory complications in two unrelated cases. Brit J Dermatol 137, 898-906.
75. Andrä K, Lassmann H, Bittner R, Shorny S, Fässler R, Propst F, Wiche G. (1997). Targeted inactivation of plectin reveals essential function in maintaining the integrity of skin, muscle and heart cytoarchitecture. Genes Dev 11, 3143-3156.
74. Eger A, Stockinger A, Wiche G, Foisner R. (1997). Polarization-dependent association of plectin with desmoplakin and the lateral submembrane skeleton in MDCK cells. J Cell Sci 110, 1307-1316.
73. Elliott CE, Becker B, Oehler S, Castañón MJ, Hauptmann R, Wiche G. (1997). Plectin transcript diversity: identification and tissue distribution of variants with distinct first coding exons and rodless isoforms. Genomics 42, 115-125.
72. Koss-Harnes D, Jahnsen FL, Wiche G, Søyland E, Brandtzaeg P, Gedde-Dahl Jr, T. (1997). Plectin abnormality in Epidermolysis Bullosa Simplex Ogna: Non-responsiveness of basal keratinocytes to some anti-rat plectin antibodies. Exp Dermatol 6, 41-48.
71. Bohn W, Etzrodt D, Foisner R, Wiche G, Traub P. (1996). Cytoskeleton architecture of C6 rat glioma cell subclones whole mount electron microscopy and immunogold labelling. Scann. Microsc. Suppl. 10, 285-293.
70. Steffen W, Hodgkinson JL, Wiche G. (1996). Immunogold-localization of the intermediate chain within the protein complex of cytoplasmic dynein. J Struct Biol 117, 227-235.
69. Nikolic B, Mac Nulty E, Mir B, Wiche G. (1996). Basic amino acid residue cluster within nuclear targeting sequence motif is essential for cytoplasmic plectin-vimentin network junctions. J Cell Biol 134, 1455-1467.
68. Smith FJD, Eady RAJ, Leigh IM, McMillan JR, Rugg EL, Kelsell DP, Bryant SP, Spurr NK, Geddes JF, Kirtschig G, Milana G, de Bono AG, Owaribe K, Wiche G, Pulkkinen L, Uitto J, McLean WHI, Lane EB. (1996). Plectin deficiency results in muscular dystrophy with Epidermolysis bullosa. Nature Genet 13, 450-457.
67. Yaoita E, Wiche G, Yamamoto T, Kawasaki K, Kihara I. (1996). Perinuclear distribution of plectin in visceral epithelial cells of rat glomeruli. Am J Pathol 149, 319-327.
66. Gache Y, Chavanas S, Lacour JP, Wiche G, Owaribe K, Meneguzzi G, Ortonne JP. (1996). Defective expression of plectin in Epidermolysis Bullosa Simplex with muscular dystrophy. J Clin Invest 97, 2289-2298.
65. Liu C-g, Maercker C, Castañón MJ, Hauptmann R, Wiche G. (1996). Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci USA 93, 4278-4283.
64. Malecz N, Foisner R, Wiche G.(1996). Identification of plectin as a p34cdc2 kinase substrate and mapping of a single phosphorylation site. J Biol Chem 271, 8203-8208.
63. Foisner R, Malecz N, Dressel N, Stadler C, Wiche G. (1996). M-phase specific phosphorylation and structural rearrangement of the cytoplasmic cross-linking protein plectin involve p34cdc2 kinase. Mol Biol Cell 7, 273-288.
62. Foisner R, Bohn W, Mannweiler K, Wiche G. (1995). Distribution and ultrastructure of plectin arrays in subclones of rat glioma C6 cells differing in intermediate filament protein (vimentin) expression. J Struct Biol 115, 304-317.
61. Foisner R, Feldman B, Sander L, Seifert G, Artlieb U, Wiche G. (1994). A panel of monoclonal antibodies to rat plectin: distinction by epitope mapping and immunoreactivity with different tissues and cell lines. Acta histochem 96, 421-438.
60. Errante LD, Wiche G, Shaw G. (1994). The distribution of plectin, an intermediate filament associated protein, in the adult rat central nervous system. J Neurosci Res 37, 515-528.
59. Wiche G. (1993). Syncolin. In: Guidebook to the Cytoskeletal and Motor Proteins, eds. Kreis TE & Vale RD. Oxford University Press/Sambrook and Tooze Publishers, Oxford, 124-125.
58. Wiche G. (1993). Plectin. In: Guidebook to the Cytoskeletal and Motor Proteins, eds. Kreis TE & Vale RD. Oxford University Press/Sambrook and Tooze Publishers, Oxford, 166-167.
57. Wiche G, Gromov D, Donovan A, Castañón M, Fuchs E. (1993). Expression of plectin mutant cDNA in cultured cells indicates role of C-terminal domain in intermediate filament association. J Cell Biol 121, 607-619.
56. Seifert G, Lawson D, Wiche G. (1992). Immunolocalization of the intermediate filament-associated protein plectin at focal contacts and actin stress fibers. Eur J Cell Biol 59, 138-147.
55. Zauner W, Kratz J, Staunton J, Feick P, Wiche G. (1992). Identification of two distinct microtubule binding domains of recombinant rat MAP 1B. Eur J Cell Biol 57, 66-74.
54. Sellner W, Oberkanins C, Stockinger L, Wiche, G. (1991). Ultrastructural localization of microtubule-associated proteins (MAP) 1 in cilia of the respiratory tract. Eur J Cell Biol 55, 248-254.
53. Wiche G, Becker B, Luber K, Weitzer G, Castañón MJ, Hauptmann R, Stratowa C, Stewart M. (1991). Cloning and sequencing of rat plectin indicates a 466-kD polypeptide chain with a three-domain structure based on a central alpha-helical coiled coil. J Cell Biol 114, 83-99.
52. Foisner R, Traub P, Wiche G. (1991). Protein kinase A- and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc Natl Acad Sci USA 88, 3812-3816.
51. Feick P, Foisner R, Wiche G. (1991). Immunolocalization and molecular properties of a high molecular weight microtubule-bundling protein (syncolin) from chicken erythrocytes. J Cell Biol 112, 689-699.
50. Foisner R, Feldman B, Sander L, Wiche G. (1991). Monoclonal antibody mapping of structural and functional plectin epitopes. J Cell Biol 112, 397-405.
49. Foisner R, Wiche G. (1991). Intermediate filament-associated proteins. Curr Op Cell Biol 3, 75-81.
48. Wiche G, Oberkanins C, Himmler A. (1991). Molecular structure and function of microtubule-associated proteins. Int Rev Cytol 124, 217-273.
47. Foisner R, Feldman B, Wiche G. (1989). Plectin-vimentin interaction: molecular binding domains and regulation by phosphorylation. In: Cytoskeletal and Extracellular Proteins: Structure, Interaction, Assembly, eds. Aebi, U., and Engel, J. Springer Series in Biophysics, vol. 3, 166-168. Springer Verlag, Heidelberg/New York.
46. Wiche G. (1989). High-Mr microtubule-associated proteins: properties and functions. Biochem J 259, 1-12.
45. Bancher C, Brunner C, Lassmann H, Budka H, Jellinger K, Wiche G, Seitelberger F, Grundke-Iqbal I, Iqbal K, Wisniewski HM. (1989). Accumulation of abnormally phosphorylated t precedes the formation of neurofibrillary tangles in Alzheimer's disease. Brain Res 477, 90-99.
44. Bancher C, Lassmann H, Budka H, Jellinger K, Grundke-Iqbal I, Iqbal K, Wiche G, Seitelberger F, Wisniewski HM. (1989). An antigenic profile of lewy bodies: immunocytochemical indication for protein phosphorylation and ubiquitination. J Neuropathol Exp Neurol 48, 81-93.
43. Wiche G. (1989). Plectin: general overview and appraisal of its potential role as a subunit protein of the cytomatrix. CRC Crit Rev Biochem Mol Biol 24, 41-67.
42. Foisner R, Feldman B, Wiche G. (1988). Partial proteolysis of plectin and localization of self-interaction and vimentin binding sites on separate molecular domains. Protoplasma 145, 120-128.
41. Foisner R, Leichtfried FE, Herrmann H, Small JV, Lawson D, Wiche G. (1988). Cytoskeleton-associated plectin: in situ localization, in vitro reconstitution, and binding to immobilized intermediate filament proteins. J Cell Biol 106, 723-733.
40. Furtner R, Wiche G. (1987). Binding specificities of purified porcine brain a- and b-tubulin subunits and of microtubule-associated proteins 1 and 2 examined by electron microscopy and solid-phase binding assays. Eur J Cell Biol 45, 1-8.
39. Foisner R, Wiche G. (1987). Structure and hydrodynamic properties of plectin molecules. J Mol Biol 198, 515-531.
38. Weitzer G, Wiche G. (1987). Plectin from bovine lenses: Chemical properties, structural analysis and initial identification of interaction partners. Eur J Biochem 169, 41-52.
37. Wiche G, Foisner R, Herrmann H, Hirt H, Weitzer G. (1987). Plectin and high-Mr MAPs: versatile connecting links of the cytoskeleton. In: The Cytoskeleton in Cell Differentiation and Development, eds. Maccioni, R., and Arechaga, J. ICSU Symp. Series vol. 8, 107-117. IRL Press, Oxford.
36. Bancher C, Lassmann H, Budka H, Grundke-Iqbal I, Iqbal K, Wiche G, Seitelberger F, Wisniewski HM. (1987). Neurofibrillary tangles in Alzheimer's disease and progressive supranuclear palsy: antigenic similarities and differences. Acta Neuropathol 74, 39-46.
35. Koszka C, Foisner R, Seyfert HM, Wiche G. (1987). Isolation of Ca2+-protease resistant high Mr microtubule binding protein from mammalian brain: characterization of properties partially expected for a dynein-like molecule. Protoplasma 138, 54-61.
34. Herrmann H, Wiche G. (1987). Plectin and IFAP-300K are homologous proteins binding to microtubule-associated proteins 1 and 2 and to the 240-kilodalton subunit of spectrin. J Biol Chem 262, 1320-1325.
33. Wiche G, Herrmann H, Dalton JM, Foisner R, Leichtfried FE, Lassmann H, Koszka C, Briones E. (1986). Molecular aspects of MAP-1 and MAP-2: microheterogeneity, in vitro localization and distribution in neuronal and nonneuronal cells. Ann New York Acad Sci 466, 180-198.
32. Artlieb U, Krepler R, Wiche G. (1985). Expression of microtubule-associated proteins, MAP-1 and MAP-2, in human neuroblastomas and differential diagnosis of immature neuroblasts. Lab Invest 53, 684-691.
31. Foisner R, Wiche G. (1985). Promotion of MAP/MAP interaction by taxol. J Ultrastruct Res 93, 33-41.
30. Briones E, Wiche G. (1985). Mr 205,000 sulfoglycoprotein in extracellular matrix of mouse fibroblast cells is immunologically related to high molecular weight microtubule-associated proteins. Pro. Natl Acad Sci USA 82, 5776-5780.
29. Zernig G, Wiche G. (1985). Morphological integrity of single adult cardiac myocytes isolated by collagenase treatment: immunolocalization of tubulin, microtubule-associated proteins 1 and 2, plectin, vimentin, and vinculin. Eur J Cell Biol 38, 113-122.
28. Koszka C, Leichtfried F, Wiche G. (1985). Identification and spatial arrangement of high molecular weight proteins (Mr 300,000-330,000) co-assembling with microtubules from a cultured cell line (rat glioma C6). Eur J Cell Biol 38, 149-156.
27. Herrmann H, Dalton JM, Wiche G. (1985). Microheterogeneity of microtubule-associated proteins, MAP-1 and MAP-2, and differential phosphorylation of individual subcomponents. J Biol Chem 260, 5797-5803.
26. Wiche G. (1985). High molecular weight microtubule-associated proteins (MAPs): a ubiquitous familiy of cytoskeletal connecting links. Trends Biochem Sci 10, 67-70.
25. Herrmann,H, Aberer W, Majdic O, Schuler G, Wiche G. (1985). Monoclonal antibody to a 43,000 Mr surface protein of a human leukemia cell line (THP-1) crossreacts with the fibroblast intermediate filament protein vimentin. J Cell Sci 73, 87-103.
24. Wiche G, Krepler R, Artlieb U, Pytela R, Aberer W. (1984). Identification of plectin in different human cell types and immunolocalization at epithelial basal cell surface membranes. Exp Cell Res 155, 43-49.
23. Wiche G, Briones E, Koszka C, Artlieb U, Krepler R. (1984). Widespread occurrence of polypeptides related to neurotubule-associated proteins (MAP-1 and MAP-2) in non-neuronal cells and tissues. EMBO J 3, 991-998.
22. Hermann H, Pytela R, Dalton JM, Wiche G. (1984). Structural homology of microtubule-associated proteins 1 and 2 demonstrated by peptide mapping and immunoreactivity. J Biol Chem 259, 612-617.
21. Herrmann H, Wiche G. (1983). Specific in situ phosphorylation of plectin in detergent-resistant cytoskeletons from cultured chinese hamster ovary cells. J Biol Chem 258, 14610-14618.
20. Wiche G, Briones E, Hirt H, Krepler R, Artlieb U, Denk H. (1983). Differential distribution of microtubule-associated proteins MAP-1 and MAP-2 in neurons of rat brain and association of MAP-1 with microtubules of neuroblastoma cells (clone N2A). EMBO J 2, 1915-1920.
19. Wiche G, Krepler R, Artlieb U, Pytela R, Denk H. (1983). Occurrence and immunolocalization of plectin in tissues. J Cell Biol 97, 887-901.
18. Wiche G, Baker MA. (1982). Cytoplasmic network arrays demonstrated by immunolocalization using antibodies to a high molecular weight protein present in cytoskeletal preparations from cultured cells. Exp Cell Res 138, 15-29.
17. Weinert T, Cappuccinelli P, Wiche G. (1982). Potent microtubule inhibitor protein from dictyostelium discoideum. Biochemistry 21, 781-789.
16. Wiche G, Herrmann H, Leichtfried F, Pytela R. (1982). Plectin: a high molecular weight cytoskeletal polypeptide component that copurifies with intermediate filaments of the vimentin type. Cold Spring Harbor Symp Quant Biol vol. 46, 475-482.
15. Wiche G, Baker MA, Kindas-Mügge I, Leichtfried F, Pytela R. (1980). High molecular weight polypeptides (around 300,000) from cultured cells and their possible role as mediators of microtubule-intermediate filament interaction. In: Microtubules and Microtubule Inhibitors, eds. De Brabander M, & De Mey J. 189-200. Elsevier/ North Holland.
14. Wiche G, Furtner R. (1980). Microtubule-associated proteins stabilize the colchicine binding activity of tubulin. FEBS Lett 116, 247-250.
13. Pytela R, Wiche G. (1980). High molecular weight polypeptides (270,000-340,000) from cultured cells are related to hog brain microtubule-associated proteins but copurify with intermediate filaments. Proc Natl Acad Sci USA 77, 4808-4812.
12. Corces VG, Manso R, de la Torre J, Avila J, Nasr A, Wiche G. (1980). Effects of DNA on microtubule assembly. Eur J Biochem 105, 7-16.
11. Wiche G, Furtner R, Steinhaus N, Cole RD. (1979). Expression of simian virus 40 gene A affects tubulin stability. J Virol 32, 47-51.
10. Wiche G. (1979). In vitro synthesis of nerve growth factor related glioma C6 cell polypeptides. Biochem Biophys Res Commun 89, 620-626.
9. Wiche G. (1979). Comparative studies of tubulin in extracts of normal and transformed cells. In: Protides of the Biological Fluids, vol 26, 523-526. Pergamon Press.
8. Wiche, G., Honig, L.S., and Cole, R.D. (1979). Microtubule protein preparations from C6 glial cells and their spontaneous polymer formation. J Cell Biol 80, 553-563.
7. Wiche G, Corces VG, Avila J. (1978). Preferential binding of hog brain microtubule-associated proteins to mouse satellite versus bulk DNA preparations. Nature 273, 403-405.
6. Wiche G, Honig LS, Cole RD. (1977). Polymerizing ability of C6 glial cell microtubule protein decays much faster than its colchicine-binding activity. Nature 269, 435-436.
5. Wiche G, Lundblad VJ, Cole RD. (1977). Competence of soluble cell extracts as microtubule assembly systems: comparison of simian virus 40 transformed and nontransformed mouse 3T3 fibroblasts. J Biol Chem 252, 794-796.
4. Wiche G, Cole RD. (1976). Reversible in vitro polymerization of tubulin from a cultured cell line (rat glial cell clone C6). Proc Natl Acad Sci USA 73, 1227-1231.
3. Wiche G, Cole RD. (1976). An improved preparation of highly specific tubulin antibodies. Exp. Cell Res. 99, 15-22.
2. Wiche G, Zomzely-Neurath C, Blume AJ. (1974). In vitro synthesis of mouse neuroblastoma tubulin. Proc Natl Acad Sci USA 71, 1446-1450.
1. Tuppy H, Wiche G. (1971). d-Aminolevulinic acid synthetase in baker's yeast. Monatshefte für Chemie 102, 1305-1310.
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