Members

Group leaders

a.o.Univ.Prof.Mag.Dr. Michael WIRTH

a.o.Univ.Prof.Mag.Dr. Franz GABOR

PhD students

Mag. Elisabeth ENGLEDER
Mag. Lukas NEUTSCH
Mag. Xueyan WANG

Diploma students

Stephanie Deinhammer
Britta Eggenreich
Astrid Fischer
Ariane Foadi
Ingrid Kaniak
Alice Kiwek
Julia Klement
Romana Koller
Michaela Kubal
Sandra Kubin
Elisabeth Lang
Bettina Luser
Regina Nowotny
Clara Pichl
Katharina Senesch
Michael Wambacher
Eva-Maria Wirth

Former members

Mag. Dr. Christian FILLAFER
Mag. Dr. Vera KERLETA
Mag. Dr. Verena PLATTNER
Mag. Dr. Gerda RATZINGER

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CELL CULTURE MODELS IN PRECLINICAL BIOPHARMACEUTICS

For preclinical biopharmaceutical evaluations, various cell culture models have been established in our group. These comprise the Caco-2 cell line, a nowadays widely accepted model for the human intestinal epithelium, models of prostate-, bladder- and mamma-carcinoma as well as human chondrocyte cell lines and the THP-1 macrophage model. Mainly, these cell culture systems are applied to elucidate preclinical biopharmaceutical aspects of specifically designed drug delivery systems. Among these our main focus is set on the estimation of cytoadhesion and cytoinvasion as well as the intracellular trafficking of drugs and their delivery systems. For a detailed characterisation of these issues, methods including flow cytometry, confocal laser scanning microscopy and tailored fluorescence techniques, particularly in the area of immunofluorescence staining for co-localisation experiments, are applied. Currently, we are developing an acoustically driven mirofluidic cell chip to analyse particle-cell interactions under shear stress. However, our expertise also encompasses traditional horizontal and vertical setups to study transepithelial transport characteristics. Moreover, in collaboration with the Technical University of Vienna microelectronic biosensors coated with viable human cells are being developed. The potential applicability of such devices primarily lies in toxicity screening of drugs and excipients, but additionally might prove to be utilisable for high throughput screening in preclinical drug research and development. Another focus in the field of cell cultures is the detailed characterisation of cell models regarding their differentiation and vitality, especially in view of the impact of surface modifications, and the underlying cell biological mechanisms.

 

Funding

1999 - 2002
Accelerated drug development by real time kinetic studies
FWF - P-13513 MED

2004
Nahrungsergänzungsmittel zur Behebung der Histaminintoleranz
FFG – Nr. 80 83 89

2004 - 2008
CellPROM – Cell programming by nanoscaled devices
EU- FP6

2007 - 2008
A formulation containing Xyloseisomerase
FFG – Nr. 816418

2008 - 2009
Caricol Resorption
FFG – Nr. 819695