Publications

Xaver M, Huang L, Chen D, Klein F.
Smc5/6-mms21 prevents and eliminates inappropriate recombination intermediates in meiosis.
PLoS Genet. 2013 Dec;9(12):e1004067. doi: 10.1371/journal.pgen.1004067. Epub 2013 Dec 26. PMID: 24385936

Woglar A, Daryabeigi A, Adamo A, Habacher C, Machacek T, La Volpe A, Jantsch V.
Matefin/SUN-1 Phosphorylation Is Part of a Surveillance Mechanism to Coordinate Chromosome Synapsis and Recombination with Meiotic Progression and Chromosome Movement.;
PLoS Genet. 2013 Mar;9(3):e1003335. doi: 10.1371/journal.pgen.1003335. Epub 2013 Mar 7.PMID:23505384

Helene Klug, Martin Xaver, Viduth K. Chaugule, Stefanie Koidl, Gerhard Mittler,Franz Klein and Andrea Pichler;
Ubc9 sumoylation controls SUMO chain formation and is central to meiotic chromosome synapsis in Saccharomyces cerevisiae;
 Molecular Cell 02 May 2013

Frese CK, Zhou H, Taus T, Altelaar AF, Mechtler K, Heck AJ, Mohammed S.
Unambiguous phosphosite localization using electron-transfer/higher-energy collision dissociation (EThcD).
J Proteome Res. 2013 Mar 1;12(3):1520-5. doi: 10.1021/pr301130k. Epub 2013 Feb 7.

Malvezzi F, Litos G, Schleiffer A, Heuck A, Mechtler K, Clausen T, Westermann S.
 A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors. EMBO J. 2013 Feb 6;32(3):409-23. doi: 10.1038/emboj.2012.356. Epub 2013 Jan 18

Schmidt A, Ammerer G, Mechtler K.
Studying the fragmentation behavior of peptides with arginine phosphorylation and its influence on phospho-site localization. Proteomics. 2013 Mar;13(6):945-54. doi: 10.1002/pmic.201200240. Epub 2013 Feb 18.

Reiter W, Klopf E, De Wever V, Anrather D, Petryshyn A, Roetzer A, Niederacher G, Roitinger E, Dohnal I, Görner W, Mechtler K, Brocard C, Schüller C, Ammerer G.
Yeast protein phosphatase 2A-Cdc55 regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4 chromatin recruitment.
Mol Cell Biol. 2013 Mar;33(5):1057-72. doi: 10.1128/MCB.00834-12. Epub 2012 Dec 28.

Cipak L, Hyppa RW, Smith GR, Gregan J.:
ATP analog-sensitive Pat1 protein kinase for synchronous fission yeast meiosis at physiological temperature.
Cell Cycle. 2012 Apr 15;11(8):1626-33.

Köcher T, Pichler P, Swart R, Mechtler K.
Analysis of protein mixtures fromwhole-cell extracts by single-run nanoLC-MS/MS using ultralong gradients.
Nat Protoc. 2012 Apr 12;7(5):882-90.

Kurzbauer MT, Uanschou C, Chen D, Schlögelhofer P. (2012)
The Recombinases DMC1 and RAD51 Are Functionally and Spatially Separated during Meiosis in Arabidopsis.
Plant Cell. 2012 May;24(5):2058-70.

Zimniak, T., Fitz, V., Zhou, H., Lampert, F., Opravil, S., Mechtler, K., Stolt-Bergner, P., and Westermann, S. (2012).
Spatiotemporal regulation of Ipl1/Aurora activity by direct Cdk1 phosphorylation.
Curr Biol 22, 787-793.

Schleiffer, A., Maier, M., Litos, G., Lampert, F., Hornung, P., Mechtler, K., and Westermann, S. (2012).
CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Nat Cell Biol 14, 604-613.

Pichler P, Mazanek M, Dusberger F, Weilnböck L, Huber CG, Stingl C, Luider TM, Straube WL, Köcher T, Mechtler K.
SIMPATIQCO: a server-based software suite which facilitates monitoring the time course of LC-MS performance metrics on Orbitrap instruments.
J Proteome Res. 2012 Nov 2;11(11):5540-7. doi: 10.1021/pr300163u. Epub 2012 Oct 22.

Holzmann J, Fuchs J, Pichler P, Peters JM, Mechtler K.
Lesson from the stoichiometry determination of the cohesin complex: a short protease mediated elution increases the recovery from cross-linked antibody-conjugated beads.
 J Proteome Res. 2011 Feb 4;10(2):780-9. Epub 2010 Nov 18.

Dudas A, Ahmad S, Gregan J.:
Sgo1 is required for co-segregation of sister chromatids during achiasmate meiosis I. Cell Cycle. 2011 Mar 15;10(6):951-5.

Edlinger B, Schlögelhofer P. (2011)
Have a break: determinants of meiotic DNA double strand break (DSB) formation and processing in plants.
J Exp Bot. 2011 Mar;62(5):1545-63.

 Gregan F, Gregan J, Skorsepa M.:
Synthesis and characterization of two homologous series of diastereomeric 2-alkoxyphenylcarbamates.
Chem Pharm Bull (Tokyo). 2011;59(8):978-83.

Gregan J, Polakova S, Zhang L, Tolić-Nørrelykke IM, Cimini D.:
Merotelic kinetochore attachment: causes and effects.
Trends Cell Biol. 2011 Jun;21(6):374-81.

Grosstessner-Hain K, Hegemann B, Novatchkova M, Rameseder J, Joughin BA, Hudecz O, Roitinger E, Pichler P, Kraut N, Yaffe MB, Peters JM, Mechtler K.
Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phosphoproteome.
Mol Cell Proteomics. 2011 Nov;10(11):M111.008540. Epub 2011 Aug 21.

Taus T, Köcher T, Pichler P, Paschke C, Schmidt A, Henrich C, Mechtler K.
Universal and confident phosphorylation site localization using phosphoRS.
J Proteome Res. 2011 Dec 2;10(12):5354-62. Epub 2011 Nov 10.

Hegemann, B., Hutchins; J.R.A., Hudecz,, O., Novatchkova,, M., Rameseder,, J., Sykora, M.M., Liu, S., Mazanek, M., Lénárt, P., Hériche, J.-K., Poser, I., Kraut, N., Hyman, A.A., Yaffe, M.B., Mechtler, K. and Peters, J.-M. (2011).
Systematic phosphorylation analysis of human mitotic protein complexes.
 Sci. Signal., 4(198) :rs12.

Cipak L, Zhang C, Kovacikova I, Rumpf C, Miadokova E, Shokat KM, Gregan J.:
Generation of a set of conditional analog-sensitive alleles of essential protein kinases in the fission yeast Schizosaccharomyces pombe.
Cell Cycle. 2011 Oct 15;10(20):3527-32. Epub 2011 Oct 15.

Hornung, P., Maier, M., Alushin, G.M., Lander, G.C., Nogales, E., and Westermann, S. (2011).
Molecular architecture and connectivity of the budding yeast Mtw1 kinetochore complex.
J Mol Biol 405, 548-559.

Köcher T, Swart R, Mechtler K.
Ultra-high-pressure RPLC hyphenated to an LTQ-Orbitrap Velos reveals a linear relation between peak capacity and number of identified peptides.
 Anal Chem. 2011 Apr 1;83(7):2699-704. Epub 2011 Mar 9.

Kurzbauer MT, Schlögelhofer P. (2011)
 Retinoblastoma protein goes green: the role of RBR in Arabidopsis meiosis.
EMBO J. 2011 Feb 16;30(4):631-3.

Lampert, F., and Westermann, S. (2011).
A blueprint for kinetochores - new insights into the molecular mechanics of cell division.
 Nat Rev Mol Cell Biol 12, 407-412.

Polakova S, Cipak L, Gregan J.:
RAD21L is a novel kleisin subunit of the cohesin complex.
Cell Cycle. 2011 Jun 15;10(12):1892-3.

Latini P, Frontini M, Caputo M, Gregan J, Cipak L, Filippi S, Kumar V, Vélez-Cruz R, Stefanini M, Proietti-De-Santis L.:
CSA and CSB proteins interact with p53 and regulate its Mdm2-dependent ubiquitination.
Cell Cycle. 2011 Nov 1;10(21):3719-30.

Whelan, G., Kreidl, E., Wutz, G., Egne, A., Peters, J.-M. and Eichele, G. (2011).
Cohesin acetyltransferase Esco2 is a cell viability factor and is required for cohesion in pericentric heterochromatin.
EMBO J. 31, 71-82.

Pichler P, Köcher T, Holzmann J, Möhring T, Ammerer G, Mechtler K.
Improved precision of iTRAQ and TMT quantification by an axial extraction field in an Orbitrap HCD cell.
Anal Chem. 2011 Feb 15;83(4):1469-74. Epub 2011 Jan 28.

Panizza, S., Mendoza, M.A., Berlinger, M., Huang, L., Nicolas, A., Shirahige, K., and Klein, F. (2011).
Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination.
Cell 146, 372-383

Batova M, Klobucnikova V, Oblasova Z, Gregan J, Zahradnik P, Hapala I, Subik J, Schüller C.:
Chemogenomic and transcriptome analysis identifies mode of action of the chemosensitizing agent CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine).
BMC Genomics. 2010 Mar 4;11:153.

Lampert, F., Hornung, P., and Westermann, S. (2010).
 The Dam1 complex confers microtubule plus end-tracking activity to the Ndc80 kinetochore complex.
 J Cell Biol 189, 641-649.

Pichler P, Köcher T, Holzmann J, Mazanek M, Taus T, Ammerer G, Mechtler K.
Peptide labeling with isobaric tags yields higher identification rates using iTRAQ 4-plex compared to TMT 6-plex and iTRAQ 8-plex on LTQ Orbitrap.
 Anal Chem. 2010 Aug 1;82(15):6549-58.

Baudrimont, A., Penkner, A., Woglar, A., Machacek, T., Wegrostek, C., Gloggnitzer, J., Fridkin, A., Klein, F., Gruenbaum, Y., Pasierbek, P., et al. (2010).
Leptotene/zygotene chromosome movement via the SUN/KASH protein bridge in Caenorhabditis elegans.
PLoS Genet 6, e1001219

Spirek M, Benko Z, Carnecka M, Rumpf C, Cipak L, Batova M, Marova I, Nam M, Kim DU, Park HO, Hayles J, Hoe KL, Nurse P, Gregan J.:
S. pombe genome deletion project: an update.
Cell Cycle. 2010 Jun 15;9(12):2399-402.

Rumpf C, Cipak L, Schleiffer A, Pidoux A, Mechtler K, Tolic-Norrelykke IM, et al. 2010.
Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4.
Cell Cycle 9(19): 3997-4004.

Rumpf C, Cipak L, Novatchkova M, Li Z, Polakova S, Dudas A, Gregan J. et al. 2010.
High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis I.
Cell Cycle 9(9): 1802-1808.

Rumpf C, Cipak L, Dudas A, Benko Z, Pozgajova M, Riedel CG, Gregan J.et al. 2010.
Casein kinase 1 is required for efficient removal of Rec8 during meiosis I.
Cell Cycle 9(13): 2657-2662.

Stasyk T, Holzmann J, Stumberger S, Ebner HL, Hess MW, Bonn GK, Mechtler K, Huber LA.
Proteomic analysis of endosomes from genetically modified p14/MP1 mouse embryonic fibroblasts. Proteomics.
2010 Nov;10(22):4117-27. doi: 10.1002/pmic.201000258. Epub 2010 Nov 2

Eils, R., Huber, W., Peters, J.-M., Hyman, A.A., Durbin, R., Pepperkok, R. and Ellenberg, J. (2010).
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Nature 464, 721-727.

Nishiyama, T., Ladurner, R., Schmitz, J., Schleiffer, A., Kreidl, E., Bhaskara, V., Bando, M., Shirahige, K., Hyman, A.A., Mechtler, K. and Peters, J.-M. (2010).
 Sororin mediates sister chromatid cohesion by antagonizing Wapl.
Cell 143, 737-749.

Morandell S, Grosstessner-Hain K, Roitinger E, Hudecz O, Lindhorst T, Teis D, Wrulich OA, Mazanek M, Taus T, Ueberall F, Mechtler K, Huber LA.
QIKS—Quantitative identification of kinase substrates.
Proteomics. 2010 May;10(10):2015-25.

Cipak L, Spirek M, Novatchkova M, Chen Z, Rumpf C, Lugmayr W, et al. 2009.
 An improved strategy for tandem affinity purification-tagging of Schizosaccharomyces pombe genes.
Proteomics 9(20): 4825-4828.

Dean PJ, Siwiec T, Waterworth WM, Schlögelhofer P, Armstrong SJ and West CE. (2009)
 A novel ATM-dependent X-ray-inducible gene is essential for both plant meiosis and gametogenesis.
Plant J. 2009 Jun;58(5):791-802.

Kiermaier, E., Woehrer, S., Peng, Y., Mechtler, K., and Westermann, S. (2009)
A Dam1-based artificial kinetochore is sufficient to promote chromosome segregation in budding yeast.
Nat Cell Biol 11, 1109-1115.

Klutstein M, Xaver M, Shemesh R, Zenvirth D, Klein F, Simchen G. (2009).
Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae.
Mol Genet Genomics 282, 453–462

Zimniak, T., Stengl, K., Mechtler, K., and Westermann, S. (2009).
Phosphoregulation of the budding yeast EB1 homologue Bim1p by Aurora/Ipl1p. J Cell Biol 186, 379-391.

Penkner AM, Fridkin A, Gloggnitzer J, Baudrimont A, Machacek T, Woglar A, et al. 2009.
Meiotic chromosome homology search involves modifications of the nuclear envelope protein Matefin/SUN-1.
Cell 139(5): 920-933.

Wendt, K.S. and Peters, J.-M. (2009).
How cohesin and CTCF cooperate in regulating gene expression.
Chromos. Res. 17, 201-214.

Mendoza-Ferri, M.G., Hartinger, C.G., Mendoza, M.A., Groessl, M., Egger, A.E., Eichinger, R.E., Mangrum, J.B., Farrell, N.P., Maruszak, M., Bednarski, P.J., et al. (2009).
Transferring the concept of multinuclearity to ruthenium complexes for improvement of anticancer activity.
J Med Chem 52, 916-925.

Mendoza-Parra, M., A., S. Panizza and F. Klein, (2009).
 “Analysis of Protein–DNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qChIP).”Scott Keeney (ed.),
 Meiosis, Volume 1, Molecular and Genetic Methods, vol. 557, Humana Press

Nativio, R., Wendt., K.S., Ito, Y., Huddleston, J.E., Uribe-Lewis, S., Woodfine, K., Krueger, C., Reik, W., Peters, J.-M. and Murrell, A. (2009).
Cohesin is required for higher order chromatin conformation at the imprinted IGF2-H19 locus.
PLoS Gen. 5, e1000739. Epub 2009 Nov 26.

Cipak L, Spirek M, Gregan J.:
Sister chromatids caught in the cohesin trap.
Nat Struct Mol Biol. 2008 Sep;15(9):899-900.

Gregan J, Spirek M, Rumpf C.:
Solving the shugoshin puzzle.
 Trends Genet. 2008 May;24(5):205-7.

Wendt, K.S., Yoshida, K., Itoh, T., Bando, M., Koch, B., Schirghuber, E., Tsutsumi, S., Nagae, G., Ishihara, K., Mishiro, T., Yahata, K., Imamoto, F., Aburatani, H., Nakao, M., Imamoto, N., Maeshima, K., Shirahige, K. and Peters, J.-M. (2008).
Cohesin mediates transcriptional insulation by CTCF.
Nature 451, 796-801.

Peters, J.-M., Tedeschi, A. and Schmitz, J. (2008).
 The cohesin complex and its roles in chromosome biology.
 Genes Dev. 22, 3089-3114.

Jordan, P. W., F. Klein, and D. Leach (2007).
 "Novel Roles for Selected Genes in Meiotic DNA Processing.”
PLoS Genet 3(12): 2368-2380.

Uanschou, C., T. Siwiec, et al. (2007).
"A novel plant gene essential for meiosis is related to the human CtIP and the yeast COM1/SAE2 gene.”
Embo J 26(24), 5061-70